Journal: The Journal of steroid biochemistry and molecular biology

Article Title: Deletion of JNK2 prevents vitamin-D-deficiency-induced hypertension and atherosclerosis in mice

doi: 10.1016/j.jsbmb.2017.09.014

Figure Lengend Snippet: JNK2−/− LDLR−/− and LDLR−/− mice were fed vitamin D-sufficient or deficient high fat diet for 10 weeks (n=4M/4F mice per group). Peritoneal macrophages harvested after HFD were assessed for (A) flow cytometry of membrane expression of M1 macrophage markers (CCR7 and CD86) and M2 markers (MR and CD163) and (B–D) mRNA expression of macrophage cytokines. Data are expressed as mean ± SEM, and horizontal lines demonstrate comparison between individual groups where *p≤0.05, **p≤0.01, ***p≤0.001.

Article Snippet: Macrophage cell surface marker analysis was performed using a FACStar Plus with PE-conjugated anti-CCR7 and anti-CD86 (E-Bioscience) for M1 macrophage membrane protein expression and FITC-conjugated anti-CD163 (Bioss USA) and anti-MR (R&D Systems) for M2 macrophage membrane protein expression in unstimulated peritoneal macrophages collected from HFD-fed mice [ 28 , 39 ].

Techniques: Flow Cytometry, Expressing